Identification of a Human Lactoferrin-Binding Protein in Gardnerella vaginalis
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چکیده
منابع مشابه
Identification of a human lactoferrin-binding protein in Gardnerella vaginalis.
Previous studies have shown that Gardnerella vaginalis can utilize iron-loaded human lactoferrin as a sole source of iron. In this study, G. vaginalis cells were shown to bind digoxigenin (DIG)-labeled human lactoferrin in a dot blot assay. Using the DIG-labeled human lactoferrin, a 120-kDa human lactoferrin-binding protein was detected by Western blot analysis of G. vaginalis proteins. The lac...
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Different tests for the identification of Gardnerella (Haemophilus) vaginalis and for its differentiation from catalase-negative unclassified coryneforms from the vagina were evaluated on over 200 bacterial strains, with special emphasis on optimal test conditions. A presumptive identification of G. vaginalis in the clinical laboratory can be made on the basis of colonial morphology, clear beta...
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A simple and reproducible scheme for identifying biotypes of Gardnerella vaginalis has been developed, based on reactions for lipase, hippurate hydrolysis, and beta-galactosidase. Among a total of 359 strains tested, eight biotypes were observed, the most common ones being types 1 (beta-galactosidase positive, lipase positive, hippurate positive), 2 (beta-galactosidase negative, lipase positive...
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With respect to paragraph 6 of Dr. L0vborg's letter, the solid phase was blocked with BSA and Tween as described. The assessment of the plates did not occur in only a few experiments. We worked with the Nunc plates using the complete ELISA procedure as described for many months before evaluating other types of plates. Finally, the view held by Dr. L0vborg in paragraph 3 that detergent is a weak...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2000
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.68.6.3443-3447.2000